Sentence examples for primer marked with from inspiring English sources

Primers used in the qPCR with the forward primer marked with 6-carboxyfluorescein (FAM) (Additional file 1: Table S1) were used for the T-RFLP (Ying et al. 2010).

A subset of samples was genotyped using newly designed primers (marked with the suffix -m).

Primers 15 and 16 amplify the region encompassing the mutation; the reverse primer is marked with 5'HEX.

3 Discrepancies were present for various pre-amplification primer combinations (marked with dashed lines in Fig. 2), with more frequent homozygosity found with the TaqMan assay; this suggested the preferential amplification of only one allele in some individuals.

The forward primers were all marked with fluorescent dye (FAM, HEX and TAMRA).

Primers marked * were designed by PrimerBank.

One primer from each pair was marked with fluorescent dye (FAM or VIC).

Forward primers of each pair were marked with 6-FAM, VIC, NED, or PET fluorescent dyes (Table 1).

The nucleotide sequences obtained were analysed using the similarity search BLAST program and subsequently used for designing gene specific primers (GSPs).> -wrap-foot> *Primarkedarked with star were provided with the kits.

Further optimization of the pre-amplification step identified primer combinations that correctly identified all genotypes (marked with solid lines in Fig. 2).

Mega-amplicons from influenza virus were internally marked with these dual-barcoded primers, which enabled multiplexing and simultaneous detection of different subtypes in the same run.