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Overrepresented motifs located four to eight bases upstream of the TSS were manually aligned with BioEdit 7.0, and this alignment was used to build a weight matrix of 12 columns.
Symbiodinium sequences were manually aligned with the BioEdit version 5.0.9 sequence alignment software [ 35] and phylogenetically analyzed using statistical parsimony implemented in the program TCS version 1.21 [ 36].
In this "template-based" meshing approach, the baseline FE (tetrahedral) volume mesh is first manually aligned with the follow-up images.
American YFV complete genome and prM/E sequences were manually aligned with reference YFV sequences from Africa and with vaccine strains obtained from GenBank and subsequent subjected to Maximum Likelihood (ML) phylogenetic analysis.
Mitochondrial sequences were manually aligned with the revised Cambridge Reference Sequence [39].
Additionally, all sequences were manually aligned with the above described reference database using PAUP* 4.0b10 [55].
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Once we identified sequences that putatively belonged to the T2 RNase gene family, we translated them to proteins, manually aligned them with amino acid sequences from verified members of the T2 RNase gene family using Se-Al, and inspected their protein-coding motifs for homology with verified members of the gene family.
Data with the control lower and upper limits were automatically called or manually aligned (see figure) with the Agilent 2100 Expert Software.
It's important to note, once again, that this is just concept footage; Foxwell manually aligned the images with the digital panoramas, which themselves were hand-scanned and very limited in scope; he connects the digital realms he captured with using hyperlapse footage, which helps SiteSeer transcend the label of mere concept video into something of a standalone short film.
In addition, we manually aligned shorter contigs with low sequence representation to the larger contigs.
The stored MRI data set was manually aligned and automatically fused with the real-time US, overlaying the ROIs on the virtual 3D prostate model (nonrigid registration).
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