Sentence examples for extracted with use from inspiring English sources

Mycelia cultured on malt extract agar were harvested with a spatula, and DNA was extracted with use of a PrepMan® Ultra Reagent (Life Technologies, Carlsbad, California, USA).

The unsaponified lipids were extracted with use of n-hexane (20 ml×2) and dried in a vacuum.

Total RNA was extracted with use of TRI Reagent (Sigma-Aldrich, St . Louis MO, USA) from 1 g inoculated leaves at 8 dpi and systemic leaves at 20 dpi from N. benthamiana plants or inoculated leaves at 7 dpi from A. thaliana.

The acetylated phytosterols were extracted with use of n-hexane (2 ml×3), and analyzed by GC-MS under the following conditions: Hewlett-Packard GC-MS (6890-5973); columnary column, HP-5 (30 mm0.25 mm i.d., 0.25 µm film thickness, J&W Scientific); column temperature 150°C for 4 min, thermal gradient 10°Cmin−1 to 280°C, and then 280°C; carrier gas helium, with flow rate, 1 ml/min.

Signal intensities were extracted with use of Feature Extraction v9.5.3.

Iso- and neuroprostanes from human plasma and urine are firstly extracted with use of SPE.

Total RNA was extracted with using TRIzol reagent (Invitrogen, Carlsbad, CA) and complementary DNA was synthesized from RNA with the SuperScriptTM First-Strand Synthesis System (Invitrogen, Carlsbad, CA).

Metabolites were extracted with the use of accelerated solvent extraction with polar (methanol + water, 80 + 20 by volume) and nonpolar (methanol + dichlormethan, 40 + 60 by volume) solvents.

Promoter luciferase assays were performed with the Dual-Luciferase Reporter Assay System (Promega, Madison WI), Twenty-four hours after transfection, cells were extracted with the use of a passive lysis buffer; a 20-µl aliquot was used for luminescence measurements with a luminometer.

Genomic DNA was extracted with the use of the DNeasy Tissue Kit (Qiagen, Valencia, CA), and sodium bisulfite conversion was performed with the use of the EZ DNA Methylation Kit (Zymo Research Corporation, Orange, CA).

Soon afterward, articles appeared in the Gulen-affiliated media saying that their confessions had been extracted with the use of hypnosis.