Sentence examples for extracted with using from inspiring English sources

Total RNA was extracted with using TRIzol reagent (Invitrogen, Carlsbad, CA) and complementary DNA was synthesized from RNA with the SuperScriptTM First-Strand Synthesis System (Invitrogen, Carlsbad, CA).

Mycelia cultured on malt extract agar were harvested with a spatula, and DNA was extracted with use of a PrepMan® Ultra Reagent (Life Technologies, Carlsbad, California, USA).

The unsaponified lipids were extracted with use of n-hexane (20 ml×2) and dried in a vacuum.

Total RNA was extracted with use of TRI Reagent (Sigma-Aldrich, St . Louis MO, USA) from 1 g inoculated leaves at 8 dpi and systemic leaves at 20 dpi from N. benthamiana plants or inoculated leaves at 7 dpi from A. thaliana.

The acetylated phytosterols were extracted with use of n-hexane (2 ml×3), and analyzed by GC-MS under the following conditions: Hewlett-Packard GC-MS (6890-5973); columnary column, HP-5 (30 mm0.25 mm i.d., 0.25 µm film thickness, J&W Scientific); column temperature 150°C for 4 min, thermal gradient 10°Cmin−1 to 280°C, and then 280°C; carrier gas helium, with flow rate, 1 ml/min.

Signal intensities were extracted with use of Feature Extraction v9.5.3.

Iso- and neuroprostanes from human plasma and urine are firstly extracted with use of SPE.

In brief, the freeze-dried <63-μm fraction of the suspended matter was extracted with toluene using Soxhlet extraction.

All samples were spiked with 400 ng of surrogate recovery standard (diphenyl isophthalate) and extracted with dichloromenthane using Soxhlet extraction apparatus.

Fat was extracted with ether using a Soxhlet apparatus and weighed following AOAC official method 920.39.

Genomic DNA was extracted with chloroform using standard procedures [ 28].