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The finely ground plant materials were extracted with ethanol for three days, and extraction of the same materials was repeat two more times.
The well-ground samples were extracted with ethanol for 12 h followed by repeated washings to remove extractives.
Reduced glutathione (GSH) from fermentation broth of Saccharomyces cerevisiae was extracted with ethanol without disruption of the cells.
Supercritical fluid antisolvent fractionation was used to obtain antioxidant compounds, mainly carnosic acid (CA), from high viscous oleoresins derived from dried rosemary leaves (Rosmarinus officinalis) extracted with ethanol.
Finally, they were extracted with ethanol for 8 h[55].
Air dried fruits were extracted with ethanol and fractionated into four fractions.
The obtained solid residue was extracted with ethanol and acetone until the eluate was colorless, respectively.
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After homogenization, the samples were freeze dried, and fat was extracted with ethanol-toluene (15/85%%, v/v) using Accelerated Solvent Extractor (Dionex ASE 300) equipment.
Concisely, samples of W. somnifera were powdered and extracted with ethanol-water (50 50; v/v) with magnetic stirring at room temperature (25 ± 2°C).
The samples were then reprecipitated with 10%% TCA (final concentration) and subsequently extracted with ethanol-ethyl acetate (1 1, v/v) and then reprecipitated at 10%% TCA.
After homogenization the samples were freeze dried and fat was extracted with ethanol-toluene using Accelerated Solvent Extractor (ASE 300) equipment.
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