Exact(8)
The filtrate was extracted with ether (2 × 20 mL).
The resulting mixture was extracted with ether and eluted with an Extrelut column.
The resulting mixture was extracted with ether and eluted from an Extrelut column (Merck, Darmstadt, Germany).
Fat was extracted with ether using a Soxhlet apparatus and weighed following AOAC official method 920.39.
The basic layer was separated, acidified with dilute HCl and a precipitate was formed which was extracted with ether.
The fatty acids were extracted with ether and esterified with 0.95 ml methanol and 0.05 ml of 98%H2SO44 at 65°C for 6 h.
After cooling, the solution was acidified with 50% HCl and the precipitate was extracted with ether (4 × 25 mL), washed with water then with dilute potassium hydroxide.
The aglycones were then extracted with ether.
Related(1)
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