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Rabbit ES cells and human ES cells display morphological and molecular similarities [15].
These controls were used to build a statistical model to distinguish ES cells from non-ES cells in PGK-clones.
Mouse ES cells are considered naïve while human ES cells resemble primed epiblast stem cells.
Moreover, ES cells expressing DNMT1 protein at ∼10% of normal concentration restored genomic methylation in Dnmt1-null ES cells [26].
Further, we compared telomere lengths of Hdac6 overexpression ES cells with those of WT ES cells.
The targeting plasmid was transfected into ES cells by electroporation.
huES9 ES cells were used as an mCherry negative control.
Also, Hdac6 overexpression ES cells expressed lower γH2AX protein levels than did WT ES cells (Fig. S1C).
We compared the chondrogenic differentiation of MS and ES cells.
The ES cells should be split every second day.
Spontaneous differentiation of ES cells into cardiomyocytes is however limited.
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