Headlong's new piece, "Cell," choreographed by Andrew Simonet, Amy Smith and David Brick, was one of the festival's most talked-about works.
In addition, AWS is looking at ways to break down those S3 sub-systems, which were core to the problem, into much smaller pieces or cells, as they call them, something they have tried to do in the past.
Tissue pieces or cells were incubated in DMEM (without phenol red) containing 1% fatty acid free BSA with or without 1 µM isoproterenol as indicated.
HFL tissue was minced into small pieces, then cells harvested by digesting with 5 mg/ml collagenase type D (Roche, Indianapolis, IN) in buffer (per 100 ml: 0.39 g NaCl, 0.05 g KCl, 0.24 g HEPES, 0.07 g CaCl2.2H20) at 37°C for 30 minutes with gentle agitation.
As to the lymph nodes, these were cut into small pieces and cells were flushed out with PBS.
While explants diced into small pieces accentuate cell apoptosis and matrix degradation, the large explants used here minimize cell death and enable maintenance of normal matrix metabolism; thus, native collagen architecture is retained away from the cut edges of the disks.
To remove any remaining tissue pieces, the cell suspension was filtered through a stainless steel mesh attached to a 60-mm cell culture plate.
Titled "Adam and Eve Swimming Under a Microscope," those pieces evoke cell division or, more ominously, viral replication, and bring to mind the work of creators as unalike as the Indian artist Bharti Kher or the British duo Gilbert & George.
Stacking of the monolithic pieces, increasing cell density, and gas redistribution between pieces reduced this distribution and improved the breakthrough performance.
We used sterile shim-stock to cut out individual pieces of cell-bearing agar and transfer them to the appropriate wells on the surface of the CD.
