As the absolute numbers of attached cells varied from donor to donor but the ratio of attached cells between the three substrates was always similar, we normalized the number of attached cells.
Three hours after irradiation, floating and attached cells were collected and washed.
The attached cells could be recovered simply by lowering culture temperature.
In the prior works, the attached cells onto plastic or activated carbon media were applied.
Floating and attached cells were pooled for all analyses.
Cells were stained with DAPI, fixed and attached cells enumerated.
Floating and attached cells were combined and harvested by centrifugation.
Thin films of attached cells were prepared as described previously with modifications [9].
Floating and attached cells were collected and combined and flow cytometry performed.
Following TQ treatment, attached cells were washed once with phosphate buffered saline (PBS).
Following the adhesion experiments, each well was triple washed with PBS to remove loosely attached cells.
