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Two days after transfection, we scored cells containing two or more distinct nuclei indicating defective mitosis (Figure 6).
For these experiments we scored cells from dissociated hindgut/genital ridge prior to cell sorting as a control.
ncell, nROI and nexp, represent the total number of scored cells, total number of detected ROIs and the total number of independent experiments, respectively.
Therefore, independently of cell speed, we scored cells as motile only if they had a maximum displacement of >25 µm, the average cell radius, at some point during the course of the assay.
We scored cells with the exception of irradiated cells.
The strongly positive descriptor (ezrin overexpression) was assigned to '++' and '+++' scored cells.
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Each dot represents a single scored cell.
We then scored cell numbers using the In Cell Developer Toolbox (GE Healthcare).
Stress fiber induction was quantified by scoring cells for the presence of stress fibers and is represented as an index of stress fiber induction in Figure 2B.
Cell apoptosis was determined by scoring cells displaying pycnotic nuclei.
Apoptosis assay Apoptosis was determined by scoring cells displaying pycnotic nuclei (visualised with Hoechst 33342) [ 13].
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