These were included to ensure specificity in antigen antibody interactions in the experiments.
Primers were designed for two variable regions of the NRPS gene sequence to ensure specificity for the emetic strains.
However, antibodies derived from in vitro selection or maturation lack an in vivo-like control system and rely on the counter screen to ensure specificity.
Amplification was performed in a thermal cycler (Appendorf) using a high stringency PCR protocol with high annealing temperature to ensure specificity of amplification.
Our results demonstrate affinity and avidity of targeting moieties on nanoparticles as important design parameters to ensure specificity and avoid toxicities.
Primers were located in exon 1 and 2 respectively to ensure specificity for cDNA.
Primers were BLAST searched against P.falciparum and human databases to ensure specificity.
The output from the ArrayOligoSelector was also analyzed using BLAST 2.2.9 to ensure specificity.
In addition, the number of amplification rounds was limited to three to help ensure specificity.
The resulting sequences were evaluated using NCBI BLAST to ensure specificity.
Staining was verified using the appropriate positive and negative controls to ensure specificity of staining.
