Exact(4)
Primers and probe are listed in Table 5. Quantitative two-step RT-PCRs were established (specificity, sensitivity, efficiency) for ORF73, Ov9, and intergenic RNA as described previously [82].
To enable immunohistochemical analysis of NAV3, we generated a monoclonal antibody, established specificity and analyzed human breast cancer specimens.
Similarly, in a more expansive study, the Mkl family ATPases fell into the SBP-dependent importer clade, but clustered separately from those with established specificity [ 8].
High HSP72 immobilization densities were used to established specificity of binding between MV N peptide and HSP72 (data not shown; see reference 15).
Similar(56)
To describe an event analysis (EA) for monitoring Heidelberg Retina Tomograph (HRT) progression and to establish specificity, detection rate, and agreement with visual field progression by application to longitudinal data.
Melt curve analysis (50°C to 99°C were performed to establish specificity of amplification).
The results suggest mechanisms establishing specificity and order in Sumo conjugation cascades.
Thus, several structural differences between E1ufds, and corresponding distinctions between their cognate E2s, may help establish specificity of E1-E2 interactions.
Calpain activity was measured in live dermal MVECs with an established fluorescent calpain substrate 7-amino-4-chloromethylcoumarin, t-BOC-L-leucyl-L-methionine amide (CMAC, t-BOC-Leu-Met; from Invitrogen) [66]; and calpain inhibitors, as indicated, were used to establish specificity.
To establish specificity of the primers for the HB-1 and ACC-1 alleles, mismatches to the gene sequences were introduced in the primer sequences (shaded nucleotides in Table 1).
In order to establish specificity of the observed effects for PDGFRβ-mediated signaling we additionally used an inhibitor of the PDGF-receptor kinase (AG1296, Merck) for the proliferation and migration assay at a concentration of 1 µM following manufacturer's instruction.
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