Sentence examples for verify specificity from inspiring English sources

We analysed melting curves of reaction products to verify specificity of primer binding and thereby circumvent any issues of non-specific SYBR green fluorescence.

A melting curve was obtained to verify specificity of the PCR.

PCR products were analyzed by agarose gel electrophoresis to verify specificity of the products.

Isotype and secondary antibody controls were performed for each staining to verify specificity.

To verify specificity of pharmacological agents, we downregulated BK and IK1 channels in U251 cells using gene-specific siRNAs.

Melt curve analyses were performed for all SYBR Green PCR amplifications to verify specificity of the reaction.

Rat primers were designed using NCBI Primer BLAST software, which verifies specificity.

PCR products were sequenced to verify the specificity of the reaction.

To verify the specificity of the developed method, standards and samples were analyzed simultaneously.

The melting curve was used to verify the specificity of the real-time qPCR reaction.

Amplification products were visualized on 1% agarose gels to verify the specificity of probe-template binding.