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However, some cross-amplification did occur at an annealing temperature of 60 °C (lower than the optimal 63 °C) or late in PCR cycling (i.e., Cq values greater than 35) indicating the need to adhere to stringent cycling conditions to maintain specificity.
Meanwhile, selection acting to maintain specificity for a different hormone that was structurally similar to aldosterone meant that once aldosterone arose, it could bind to the mineralocorticoid receptor which remained similar to the ancestral form (a process Bridgham et al. 2006 call "molecular exploitation"; Fig. 1A).
The 3.5 score threshold was used to maintain specificity, although at the cost of sensitivity, in the target prediction algorithm.
Pre-treatment of BMSC with U0126 and SB 203580 in concentrations held low enough to maintain specificity of inhibition resulted in approximately a 50% decrease of IL-6 upon exposure to NGF or BDNF (Figure 7).
Variation in the primer sites was necessary to maintain specificity for each gene.
To maintain specificity and sensitivity, TMAP implements a two-stage mapping approach.
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Reported antigen MAL5 was recognized with IgA having 82.6% sensitivity with maintaining specificity up to 54.5% which is the best among all these specific antigens.
Sensitivity needs to be increased while maintaining specificity for actual use in an environment with varying antibiotic prescription practices.
In addition, at least one mAB maintained specificity for anandamide compared to two very closely related fatty acid amide molecules.
We suggest that experimentally observed internal states of the Ago-miRNA search complex may have the explicit biological function of speeding up search while maintaining specificity.
The sensitivity of such clinical predictive rule was markedly improved (66%) with a maintained specificity (87%) by taking into account the presence of Candida colonization at time of its assessment [74].
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