Thus, we studied the effect of the assumption that all genes would be affected similarly by the differential differentiation efficiency.
For instance, hiPSC lines have been shown to differentiate into neuronal lineages with variability in differentiation efficiency and in electrophysiological properties (Hu et al., 2010).
LY-treated cells showed reduced differentiation efficiency, whereas both ADE and recovered LY-treated ADE efficiently generated hepatocytes.
However, the necessity for further improvement in differentiation efficiency remains.
This study aims to build silk-CNT composite scaffolds for improved neuron differentiation efficiency from hESCs.
Among all, TGFβ1/BMP4 combination had the highest differentiation efficiency, based on immunohistochemical analyses.
However, the current myogenic potential of ASCs is limited by their low differentiation efficiency into myoblasts.
The differentiation efficiency was characterized using flow cytometry, immunohistochemistry staining, and trilineage differentiation.
Their low differentiation efficiency, however, limits further application of MSCs in clinical therapy.
The tMP surface was examined for its differentiation efficiency using mouse mesenchymal stem cells, C3H10T1/2.
Two neuronal markers (β-III tubulin and nestin) were used to further determine the hESC differentiation efficiency.
