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Medium was replaced with fresh differentiation medium every day.
Medium was replaced with fresh differentiation medium every 2 days.
For RNA FISH, THP-1 cells, or CD34+ PB HSPCs differentiated for 11 days in granulocytic differentiation medium were used.
The next day, differentiation medium (DMEM high glucose, with 2% horse serum) was added to the cells (conditioned differentiation medium).
Two days later, the conditioned differentiation medium of C2C12 was replaced by new conditioned differentiation medium from freshly transfected 10T1/2 cells (differentiation condition day 2).
Half of this differentiation medium was changed every 3 days during differentiation.
Cells were grown in differentiation medium for the number of days indicated in each experiment.
Each differentiation medium was replaced every 2 3 days for 21 days.
Cells were plated in differentiation medium for four hours and then monitored.
In brief, piPSCs were digested and suspended in differentiation medium without 2i/LIF.
After 2 days, the culture medium was changed to Cardiomyocyte Differentiation Medium B containing Wnt inhibitor and the medium was changed to fresh Cardiomyocyte Differentiation Medium B every other day for an additional 6 days.
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