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Human and chicken Salmonella isolates were serotyped and phage typed by using published methods (25 – 25 ).
Campylobacter organisms were isolated and identified to species by using published methods (7).
Lung tissue was screened for viral respiratory pathogens by real time reverse transcription PCR by using published methods (7 ).
We used viral surveillance data to estimate the proportion of influenza-positive specimens if all had been tested by using published methods (9 ).
Relationships among isolates were inferred from Rep-PCR genotypes by using published methods that maximize the correspondence of such inferences to the standard of multilocus sequence typing (30 ).
Simplex and multiplex PCRs were performed by using published methods specific for ctxA, tcpA, rtxA, rtxC, stn, and hlyA genes (classical/El Tor) and the TTSS-coding genes (7 ).
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Australia's Carbon Farming Initiative is a world-leading policy that allows carbon credits to be obtained by using published methods-based approaches.
Two additional SSR loci were obtained via a genomic library enriched for CAG repeats with target clones identified by PCR using published methods (Adcock et al., 2005).
We then calculated Disability Adjusted Life Years (DALYs) saved as the sum of the Years of Life Lost (YLL) and the Years Lost due to Disability (YLD) that would be saved for each HIV infection averted by each prevention intervention using published methods [ 11- 14].
To determine if the two coprolites (Z1 and Z2) represented separate individuals, Native American mitochondrial haplogroups were identified by PCR and gel electrophoresis using published methods [32].
Sessions were recorded and transcribed, then themed by all three authors independently using published methods [ 20].
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