Sentence examples for by using published protocols from inspiring English sources

Samples of selected cultures at various passage levels and the final subcultures after >30 passages were examined for PrPTSE by WB by using published protocols (14 ).

After euthanasia, brains were removed and portions stored frozen for WB (22 ) or in 10% formalin solution for histopathologic and immunohistochemical studies by using published protocols (8 ).

Thus, we assessed presence of PrPSc in skeletal and heart muscle by Western blot analysis with sodium phosphotungstic acid precipitation for enrichment of PrPSc and protein misfolding cyclic amplification by using published protocols (3 ).

Cellular membranes from 4 × 15 cm confluent dishes of untreated, siRNA treated or Latrunculin treated cells were separated by density using published protocols (Smart et al., 1995).

The SNPs A986S and R990G located in the exon 7 of CaSR gene were analyzed by RFLP-PCR using published protocols [ 14].

Heat-inactivation of citrullinating capability was confirmed by a colorimetric assay using published protocols [ 31].

Here, human NLP showing only three major bands (Fig. 13C, lane 2) were first contrasted with the smear of bands seen with NB obtained by inoculation of 10% HS into DMEM using published protocols as before (Fig. 13C, lane 1). Figure 13C shows that these same NLP could be made to acquire many other protein bands when inoculated into DMEM containing 10% HS (lanes 3 7).

Microarray analysis was performed using arrays provided by JCVI/PFGRC under the NIAID contract N01-AI-15447 using published protocols [23].

Plant nuclear DNA isolations were performed using published protocols [[ 2]].

Plasmid was isolated from DH5α containing pCAMBIA1302 using published protocol [15].

VO2maxwas assessed on a bicycle ergometer (Ergoline Gmbh, Lindenstr, Germany) using published protocol [25].