The cellular fractionation protocol was adapted from published methods [ 31].
High information-content fingerprinting (HICF) was adapted from published methods [ 99].
Most of these assays were adapted from published methods that had independently been developed, validated and subjected to peer review.
Histochemical methods were adapted from previously published methods from our lab [50], [17], [18], [20].
The crosslinking and immunoprecipitation procedure was adapted from previously published methods [14].
Fluorescence microscopy and image analysis procedures were adapted from previously published methods [ 25, 34, 35].
The method for polysome isolation and analysis for P. pastoris was adapted from previously published methods [ 6, 19].
The extraction method for transporter proteins was adapted from previously published methods 23 and is described in Data S1.
The analytical method for measuring DINP oxidative metabolites in urine was adapted from previously published methods (Blount et al. 2000; Silva et al. 2003, 2004b).
Mammalian-cell-derived PUF60 and U2AF65/35 were expressed in 293E cells transiently transfected with pTT3-HisPUFS or pTT3-HisU2AF35 in a procedure adapted from a published method [60].
