Sentence examples for by using established methods from inspiring English sources
Recombinant DNA techniques were performed by using established methods [46] or following the instructions supplied with specific reagents.
To characterize carbapenem-resistant and mHT-positive isolates, we used PCR to amplify and sequence the carbapenemase genes blaIMP, blaNDM, blaVIM, and blaKPC by using established methods (6 ).
HIV status was determined by using established methods, and children positive for HIV were referred to the Mulago Hospital Pediatric Infectious Disease Clinic for further care.
Ex vivo indexes of cardiac function were then determined in isolated-perfused working heart preparations by using established methods [ 5, 25].
The antibiograms were consistent for isolates from all 30 patients; MICs of selected agents for a representative isolate are shown in Table 2. *MICs were determined by serial agar dilution by using established methods (24 ).
To define the relative lesion bypass efficiency of each Y-family polymerase by using established methods, the percent of lesions bypassed by each polymerase with respect to the total number of lesions encountered (8-oxodG bypass%) within the autoradiograms shown in Figure 1 was plotted as a function of reaction time.
In order for our methodological approach to be acceptable by systematists using established methods, we developed techniques that allowed us to sample sufficient taxa and perform the analysis quickly.
Presence of genetic markers associated with penicillin resistance (blaZ) and methicillin resistance (mecA) were tested by PCR using established methods [ 26, 27] for all isolates selected for strain typing, and these isolates were also screened for beta-lactamase expression using a nitrocefin assay [ 28].
Master molds for the microfluidics based wounding device were created using silicon wafers and layer-by-layer photolithography using established methods (Ferry et al., 2011).
The trends of the results obtained with the drop test method are similar to those shown in studies by other researchers using established methods like the AFM and the centrifuge method.
Primary cultures of human airway epithelia were prepared from nasal tissue and bronchi by enzymatic dispersion using established methods [52].
