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Genomic DNA was extracted from 2 ml frozen whole blood or bone marrow using the RelaxGene Blood DNA system according to the manufacturer's protocol (Tiangen, China).
Additionally, RD114 provirus was not detected in genomic DNA extracted from peripheral blood, lymph nodes, spleen and sternal bone marrow, using the one-step PCR assays.
MSCs or skeletal stem cells were enriched from human bone marrow using the STRO1 antibody and magnetic activated cell sorting (MACS) as previously described [2].
In other words, a 42-fold increased yield of early-stage BM-MSCs could be produced from bone marrow using the gelatin matrix.
Mesenchymal stem cells were isolated from human donor red cell-enriched rib bone marrow using the RosetteSep human MSC enrichment cocktail and cultured as defined by Gazi et al (2007).
Bone marrow was obtained at the end of the experiments from a number of randomly selected mice and used to determine the in vitro sensitivity of the grafted bone marrow using the in vitro bone marrow toxicity test.
Similar(54)
This suggests a role for RT-PCR in the detection of colon carcinoma metastasis in blood and bone marrow, using CK 20 as the target gene.
The activities administered were based on an estimate of the maximal tolerable dose to bone marrow, using historical 177Lu-BR96 biodataribution data for the theoretical calculation of the absorbed dose resulting from treatment with 211At-BR96 [27].
Another aspect is the possible difference in the kinetics of the radiopharmaceutical in the low uptake compartment compared to the bone marrow, which may lead to an overestimation or underestimation of the absorbed dose to the bone marrow using a fixed ratio.
Mast cells were immunomagnetically isolated from the peritoneal lavage and from the bone marrow using mAb AA4 coupled to tosylactivated Dynabeads M-450 as previously described [25], [26].
Thirty-five patients with prostate cancer were examined for micrometastases to the bone marrow using reverse transcription-polymerase chain reaction (RT-PCR) with primers specific for the prostate-specific antigen (PSA) gene.
Related(20)
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