BALB/c mice were immunized with IL-12p70 DNA or vector DNA and infected 4hr later with HSV-IL-2.
Empty pcDNA3.1 vector DNA was used to maintain a constant amount of total DNA per well.
Ten-fold serial dilutions of a vector DNA were used as standard for quantitation.
Transformation frequencies were in the range 36 to 87 transformants per 10 μg of vector DNA and 106 protoplasts.
As control, the pLAT52 vector DNA was also bombarded.
The lentiviral control vector DNA containing a scrambled sequence and the vector DNA for silencing human GR and human nestin were obtained from Sigma, Germany.
After 20 cycles, all free inserts and vector DNA had merged to form the complete plasmid.
The amount of DNA was kept constant for each electroporation using empty vector DNA.
The targeting vector DNA was prepared using the Qiagen Plasmid Maxiprep kit.
Transformed cell lines are selected by using the hygromycin resistance marker encoded by the vector DNA.
The distribution of vector DNA was restricted to the injected eye without detectable spreading.
