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The double stranded cDNA was digested with SfiI, size fractionated through Sephacryl - 500 column (Amersham), and directionally cloned into the SfiI predigested vector DNR-LIB (Clontech).
The 1 kb XmaI/ PpmuI restriction fragments covering highly conserved miRNA sites in Fluc-3′UTR and Fluc-EJC-3′UTR were replaced with 0.8 kb HpaI fragment from pSIREN-dsRED DNR vector (Clontech Laboratories, Inc., Saint-Germain-en-Laye, France), resulting in Fluc-3′UTRmut and Fluc-EJC-3′UTRmut constructs.
RNAi-ready pSiren-DNR-DsRed-Express vector was obtained from Clontech (Mountain View, CA, USA), which contains the RFP.
The DNR is really a fiction.
Thursday at 8 p.m. DNR, rock.
Aug. 15 at 7 p.m. DNR, rock.
Vector means carrier.
DAMIEN RICE: "O" (Vector).
Yet more Vector graphics!
4 3 VECTOR, by Robin Cook.
Vector, hector, rector?
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