Sentence examples for vector rna from inspiring English sources

PCR of an empty vector using GF200 primers (5'-CTGCAAGGCGATTAAGTTGGGTAAC-3' and 5'-GTGAGCGG-ATAACAATTTCACACAGGAAACAGC-3') yields a template for T7 based RNA amplification (MessageAmp™ T7 Linear Amplification Kit, Ambion, Austin, TX) to produce "vector RNA" (vRNA).

Primer/probe sets annealing to the front (LTR), the centre (GFP) or at the end (WPRE) of the genomic RNA of lentiviral vectors were used to determine to what extent full-length genomic vector RNA is incorporated into lentiviral vector particles.

The vector RNA can be packaged into recombinant alphaviral particles in cells via co-transfection with a helper RNA encoding structural genes (capsid and envelope).

However, spliced vector RNA was not transduced to target cells.

Our studies show that the intervening sequence in SIGA vector RNA is partially spliced.

For each mutant we compared the binding competition of labeld synthetic (vector) RNA with unlabeled cellular RNA.

Using a semi-quantitative RT-PCR approach on heterologous non-viral vector RNAs in an in vivo packaging assay, this study demonstrates that the principal packaging determinants of FIV reside within the first 150 bp of 5′ UTR and 100 bp of gag (the two core regions) and not the viral 5′ LTR.

The titer of foamy vector virions that contain this tat/rev shRNA is not adversely affected [42], so we reasoned that knockdown of lentiviral vector RNAs by cleavage of the shRNA target in the lentiviral vector was likely not the main mechanism for the low titers observed since this mechanism would also be expected to reduce foamy vector titers.

The shRNA sequences were introduced into the retroviral vector RNA-mediated interference Ready pSiren (BD BioSciences, Le Pont-de-Claix, Pont-de-Claix Pont-de-Claix, France

SAMD9 expression reduced by RNA interference vector for SAMD9 was tested by co-transfection of an N-terminal EGFP tagged SAMD9 expression vector with either one of the two SAMD9 RNA interference vectors, RNA interference vector with mutated SAMD9 sequence to null its function as negative control, or its control empty vector into Cos-1 cells.

In repeat experiments we collected 1° cell aliquots at serial time points, from 0 to 76 hours after vector exposure for real-time RT-PCR to detect cytoplasmic vector-RNA genome-specific long terminal repeats (LTR).