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Subsequent technical confirmation of the data with additional qPCR assays confirmed that miR-30a and miR-143 exhibited significantly altered expression levels after SAH when compared to sham animals.
Technical confirmation of an intermediate peak in the U.S. Dollar could be the catalyst for a breakout by CAD above 98 U.S. cents, with a target of 103 U.S. cents.
Validation of these genes by qRT-PCR provided excellent technical confirmation of the microarray results.
The selection of miR-103 for normalization was made by subjecting the data from the technical confirmation to the "Norm-finder" algorithm.
Technical confirmation of the candidate miRNAs from the miRNAome screen was carried out in triplicate using the same RNA template and primers as for the screen.
However, taken out of context, this is highly unusual; technical confirmation of results from a validated assay using an alternate methodology prior to reporting is not often employed for other types of molecular testing.
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Just twelve hours after they had landed, as Armstrong and his co-walker, Edwin "Buzz" Aldrin, powered away from the moon and back to the command module that would take the astronauts home, after he'd spelled out the technical confirmations that things were working well, he added a little exclamation: "I'm going right down U.S. 1" — part jocularity, and, we might assume, part relief.
Last week, Coincheck indicated that it would resume withdrawals of JPY after "[completing] the technical safety confirmation with the cooperation of external experts".
Interviewers look for information that supports their pre-conceived belief about a candidate (the technical term is confirmation bias).
However, this last exercise would require both the establishment of an in vitro culturing system for A. willeyana (an exercise that has many technical challenges) and confirmation that drugs developed for the modulation of the autophagy pathway in other organisms also function as expected in the deeply evolutionarily divergent A. willeyana.
"These include a lack of transparency in the bidding process, the fact that published guidance was not complied with when bids were being processed, inconsistencies in the treatment of bidders and confirmation of technical flaws in the model used to calculate the amount of risk capital bidders were asked to provide to guard against the risk of default".
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com