Sentence examples for stabilizing buffer from inspiring English sources

The political tremors are disconcerting for American policy makers because of the role Jordan has played as a dependable ally and a stabilizing buffer zone in a volatile region.

The most stabilizing buffer, sodium glutamate, and sodium acetate were determined for further investigations.

30 heads from 3 day-old flies were cut and immediately put in RNA stabilizing buffer (RNAwiz™, Ambion).

Cytoskeleton was prepared in microtubule stabilizing buffer (MSB) with the addition of protease inhibitors (Complete Mini, Roche).

Briefly, HUVECs were rapidly washed in microtubule stabilizing buffer (MSB) (85 mM Pipes, pH 6.93, 1 mM EG'I'A, 1 mM MgCl2, 2 M glycerol, and protease inhibitors: 10 µg/ml aprotinin, 0.5 mM benzamidine, 5 µg/ml o-phenanthroline, and 0.2 mM PMSF) and then extracted with MSB containing 0.05% Triton X-100.

Prior to fixation in 4% formaldehyde (pH 6.9) with 100 mM K-PIPES, 1 mM MgCl2, 0.1 mM CaCl2 and 0.1% Triton X-100, cells were incubated at 37°C in microtubule stabilizing buffer (4M glycerol, 100 mM K-PIPES (pH 6.9), 1 mM EGTA, 5 mM MgCl2, 0.5% Triton X-100).

Bicine at pH 7.0 and glycine at pH 8.2 were also stabilizing buffers, albeit within a more limited pH range (Table 1, Table S1).

Samples were immersed in stabilizing buffers containing antioxidants and stored at −80°C until DNA purification, which was performed with proteinase K and solvent extraction in an oxygen-free atmosphere, as previously described [9].

In order to detect structural changes across the stable pH regime, we next compared the secondary structure of myoc-OLF by conducting CD melts in stabilizing buffers of varying pH.

For BrdU staining, the cells were first surface stained, then fixed and permeabilized using Cytofix/Cytoperm (BD Biosciences), washed in Perm Buffer (BD Biosciences), incubated 30 min at room temperature with FITC-conjugated anti-BrdU in Perm Buffer, washed, and resuspended in 1X BD stabilizing fixative buffer (BD Biosciences) [42].

Complete disappearance of the activated phase is obtained in stabilizing glucose buffer.