Sentence examples for fixing buffer from inspiring English sources

The bacteria were centrifuged (Falcon(TM) type) at 3000g for 3 min at room temperature and fixed by mixing the suspension with a fixing buffer (glutaraldehyde 2%, paraformaldehyde 1%, phosphate buffer 0.1 M, pH = 7.4).

Subsequently, the cells were incubated with a 1 32 dilution of the FITC anti-mouse IgG at 4 °C in the dark and washed with FACS buffer to remove unconjugated antibodies and finally resuspended in fixing buffer (PBS, 1% paraformaldehyde).

The cells were washed with PBS and suspended in 20 µl of fixing buffer (1% FCS, 2.5% paraformaldehyde in PBS).

Cells were allowed to spread for 60 min in the incubator and then stopped by directly adding fixing buffer followed by washing with phosphate buffered saline (PBS).

The pellet was resuspended in 500 µl of fixing buffer (4% (v/v) formaldehyde, 0.1 M sodium cacodylate, 4.2 M NaCl, 220 mM MgCl2, 40 mM MgSO4, pH 7.0) and incubated for 10 minutes at 42°C.

Cells were centrifuged at 800Xg for 4 min, washed once with FACS buffer, and re-suspended in 50 μL fixing buffer (1% paraformaldehyde in 1X PBS).

The country's largest fixed buffer zone, 36 feet, is in effect in Melbourne, Fla.

Fig. 5 a Performance comparison for fixed buffer design (input SNR criterion).

Fig. 6 a Performance comparison for fixed buffer design (channel power criterion).

We consider fixed buffer-aided exhaustive/greedy (FBAE/FBAG) relay pair selection strategies (RPS) and dynamic buffer-aided exhaustive/greedy (DBAE/DBAG) RPS.

We then develop a mechanistic model, describing spatio-temporal changes of [H+]i in a cardiac ventricular myocyte in terms of H+-shuttling H+-shuttlingffers and H+-anchoning on fixed buffers.