Sentence examples for stabilisation buffer from inspiring English sources

G proteins were then loaded onto PD-10 columns pre-equilibrated in stabilisation buffer (50 mM HEPES-KOH, pH 7.5, 100 mM KCl, 5 mM EDTA, 10 mM MgCl2, 1 mM DTT) to remove unbound GDP.

To detect acetylated and detyrosinated (Glu-) tubulin, cells were lysed as above except that pre-warmed (37°C) MT stabilisation buffer (85 mM PIPES pH 6.9, 1 mM EGTA, 1 mM MgCl2 and 2 M Glycerol) was used instead of PBS and lysates were not incubated on ice.

Membrane filters were immediately placed in cold sterile stabilisation buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 2 M NaCl) and agitated to resuspend the cells.

Neutrophils were lysed within an F-actin stabilisation buffer containing ATP (1 mM) and protease inhibitors (as indicated above) for 10 min at 37°C.

BAL fluid, obtained from a radiologically affected lung segment, was concentrated ~10 fold before analysis whilst cell pellets were immediately fixed in RNA stabilisation buffer.

Five mg of lyophilised skin secretion was dissolved in 1 mL of mRNA stabilisation buffer and polyadenylated mRNA was isolated from this solution using magnetic oligo-dT beads as described by the manufacturer (Dynal Biotech, UK) and reverse-transcribed.

Briefly, adherent cells were extracted for 2 min on ice with cytoskeletal stabilisation (CSK) buffer (0.3  M sucrose, 0.5% Triton X-100, 100 m M PIPES pH 6.8, 100 m M KCl, 1 m M CaCl2, 2.5 m M MgCl2, 50 m M NaF plus 1 m M sodium orthovanadate, 1 m M PMSF, 1  μg ml−1 leupeptin and 1  μg ml−1 aprotinin).

The Scottish government's Fiscal Commission, in October 2013, said there was "clear merit" in an independent Scotland setting up two oil funds - a short-term "stabilisation" fund to buffer the effects of volatility in the oil market, and a long-term savings fund to ensure future generations benefited from the wealth.

Urine was collected in 20 ml universals without any stabilisation or preservative buffer.

Higher trehalose concentration may have a greater stabilisation effect, but buffer dilution and the 1.8 M solubility limit the maximum concentration in solution during CIP [ 63].

The possibility of buffer-based stabilisation of faecal tumour-M2-PK should be explored.