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By comparison, competing systems arbitrarily crop x% of the image off to use as a stabilization buffer.
The cells were incubated at 37 °C for 5 min and then stabilization buffer (50 μl) (ChemoMetec, Allerod, Denmark) was added to the mixture.
Swabs were collected into sterile tubes containing 400 μl DNA/RNA stabilization buffer (Roche Molecular Biochemicals, Indianapolis, IN, USA) and stored at − 80 °C until the DNA was extracted.
Fractions were collected from the bottom, refractive index was measured and the fractions were dialyzed against stabilization buffer.
Stabilization buffer, consisting of 20 µM Taxol (Molecular Probes) in BRB 80, was added to the microtubule polymerization reaction.
Swabs absorbed the full volume of stabilization buffer in addition to saliva, and thus required manual extraction ('squeezing' with sterile tweezers) to remove solution for use in extraction.
Similar(29)
These crystals were soaked in stabilization buffers at pH 6.8 7.2 and continued to diffract X-rays to better than 2.0 Å resolution.
The molasses effect, while consistent with other research on rumen fermentation, was less marked than expected, reflecting fermentation moderation through monensin and stabilization with bicarbonate buffer.
The concentration dependence of buffer stabilization or destabilization was further investigated using acetate buffer pH 4.6 or Tris buffer pH 7.5 as examples, respectively (Table S1).
The LB3.1 and MEM264 were regenerated with 20 mM NaOH for 1.5 or 1 minutes respectively, followed by a 2 minute stabilization in running buffer.
For immunohistochemistry pre-treatment, the FFPE and HOPE sections were deparaffinised, using EZ Preparation Buffer, pH 7.0, followed by stabilization reaction with TRIS Buffer, pH = 7.6-7.8 (VenTucsonedicAZ Systems, TUSAon, AZ, USA).
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