In contrast, random primers are used for making cDNA of all the RNA population including poly A-RNAs and ribosomal RNAs.
For example, although the RNA population being sequenced may be polyadenylated-RNA or total RNA, both experiments will be annotated to the RNA-seq assay.
The RNA population enriched in small RNAs was extracted from muscles using the mirVana miRNA isolation kit (Ambion, Austin, TX).
Introduction of high-throughput next-generation sequencing (NGS) technologies has increased the number of miRNAs identified by allowing small RNA population analysis on a massive scale (RNA-seq).
This study demonstrates that apple phloem sap contains small RNAs that are major contributors to the phloem sap RNA population.
This fraction of 21-nt phasiRNAs represents 49%% of the unannotated 21-nt small RNA population (4 418 over 8 977 sequences).
Our RNA-seq analysis revealed a dramatic difference between the two species in the 21 nucleotide small RNA population, corresponding mainly to miR2118-triggered phasiRNAsRNAsiRNAs
Northern analysis shows that TSSa-RNAs are subsets of an RNA population 20 to 90 nucleotides in length.
In total, 5 052 distinct 21-nt small RNA sequences associated with these 892 phased loci were found, corresponding to 21.2 % of the panicle-derived 21-nt small RNA population from the two species.
The 21-nt small RNA population from the two species, corresponding to 23 798 distinct sequences, exhibited a surprisingly large fraction of small RNAs with a higher expression in O. barthii than in O. glaberrima (Fig. 1a).
Deep sequencing of the small RNA population of these roots may reveal an important regulatory molecule.
