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Exact(21)

We applied the pairwise deletion to remove gaps as our sequence lengths are varied, and a complete removal of the gaps is not a good choice as it eliminates a large portion of phylogenetically meaningful sites from consideration.

(ii) Remove gaps and inframe stop codons: "Yes".

Multiple sequence alignments were generated using ClustalW [57] and edited using Bioedit [58] to remove gaps.

Full length sequences for all taxa were aligned with Muscle 3.6 [48] and edited manually in the case of clear errors and to remove gaps.

To construct phylogeny, sequences were first aligned with ClustalW, edited in BioEdit to remove gaps, and then Neighbor-joining trees were constructed and drawn with TREECON for windows.

PAL2NAL was also used to remove gaps and in frame stop codons.

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Similar(39)

The alignments were pruned to remove gap sites present in 50% or more of the aligned sequences.

Unlike the alignments used for the phylogenetic analyses, we did not remove gapped characters to avoid artificially increasing the number of PICs near the alignment origin due to shortening the alignments.

Sequences were aligned using MAFFT program [55], alignments were curated using Gblocks tool (version 0.91b) [56], removing gaps and saturated positions and thus keeping only informative sites.

Therefore, we created a sub-dataset of 80 sequences (accounting for a total of 1,987 nucleotides after removing gaps and missing data) that included only those sequences that did not produce multiple matches in none of the genomes.

MONKEY removes gaps locally, creating an ungapped local alignment for each position in the reference species.

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