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Sequences were aligned using MAFFT program [55], alignments were curated using Gblocks tool (version 0.91b) [56], removing gaps and saturated positions and thus keeping only informative sites.
Therefore, we created a sub-dataset of 80 sequences (accounting for a total of 1,987 nucleotides after removing gaps and missing data) that included only those sequences that did not produce multiple matches in none of the genomes.
After removing gaps with the pairwise-deletion option, a total of 338 informative positions were used to build NJ tree.
The parameters for running PAML were as follows: Empirical amino acid substitution model and removing gaps columns.
After removing gaps, the level of synonymous substitution was estimated using the yn00 program in PAML [ 23].
With clusters, the focus is not just on removing gaps and preventing duplication, but on jointly moving towards commonly agreed cluster objectives.
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Therefore, we calculated mutation rates per locus by multiplying the previously estimated mutation rates by the length of the Neomys alignments after removing gap positions.
We applied the pairwise deletion to remove gaps as our sequence lengths are varied, and a complete removal of the gaps is not a good choice as it eliminates a large portion of phylogenetically meaningful sites from consideration.
(ii) Remove gaps and inframe stop codons: "Yes".
Multiple sequence alignments were generated using ClustalW [57] and edited using Bioedit [58] to remove gaps.
Full length sequences for all taxa were aligned with Muscle 3.6 [48] and edited manually in the case of clear errors and to remove gaps.
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