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Exact(5)
This allowed us to identify some models as being artifactual due to misalignment or redundant alignment to known loci, excluding these from further analysis.
In detail, the nucmer program of the package was used to generate a chromosomal alignment; then the delta-filter program was used to filter the redundant alignment and the show-snps program was finally used to report the substitutions.
This could be done both toward the end of the pipeline combining the sets of hits earlier to reduce redundant alignment of sequences found by both methods and at the beginning of the pipeline, reducing the number of sequences that are subjected to the structure-based pipeline at all.
Predicted TEI sites greatly differing from the PacBio breakpoint tend to show a high variation between PacBio reads as well, indicating that these sites allow for multiple correct (redundant) alignment possibilities due for example to tandem or simple sequence repeats at the insertion site.
Since the first 33 base pairs of these five tRNAs are the same, the redundant alignment resulted in the identification of a G > A SNV in all five SerAGA tRNAs for the homologous position, when in fact it may have only been seen in as few as one of the five SerAGA isotypes.
Similar(54)
After removing the redundant alignments, we ended up with a total of 775 unique porcine miRNA candidates (Table S1).
Sequence similarity data was creating using all-against-all BLAST comparisons within the training and test data sets, and between the training and test data sets and the use case data sets (redundant alignments were excluded).
Using the sorted alignments ("maf-sort.sh" script), we finally proceeded to "maf-cull" step to remove redundant alignments.
To characterize the alignment quality and reduce the number of redundant alignments with low matching scores, three different quality indexes were defined and added to the global match file as follows: - %_ALIGN/TARG: Rough ratio between aligned portion of a potato's EST against the total length of a tomato's target (ALIGN_LENGTH/LENGTH_TOM) * 100 to roughly assess alignment regions.
The non-redundant alignment was performed manually with GENEDOC and using the aforementioned groups of amino acid similarity.
To perform this alignment we followed the same strategy used to obtain the non-redundant alignment using CAPs.
Related(20)
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