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For each member sequence of a pfam-domain query (reduced-redundancy alignment), PSI-BLAST was to scan the predicted proteome panel.
A method has been developed to measure relative chamber positions with curved muon tracks with comparable precision in order to increase the redundancy of the alignment system.
We removed the redundancy of this alignment (50%) to keep the most representative sequences from the profile.
In practice, the best BioNano assembly is selected based on similarity to the estimated haploid genome length and minimal alignment redundancy to the reference in silico maps.
Although overall alignment redundancy was rare for the S t r i c t- T assembly; when observed, it usually consisted of only two consensus genome maps aligning to the same in silico map (Fig. 9).
The S t r i c t- T A n d R e l a x e d- M i n l e n assembly had a worse cumulative length and alignment redundancy than the S t r i c t- T assembly.
We implemented several filters to lower the computational costs and the degree of redundancy among the alignments.
After removal of the first set of redundant entries, all remaining entries in the BPPD were aligned to each other using All vs. All BLAST [ 15] and all alignments with 90% or higher identity were again manually checked for redundancy using a ClustalW alignment and our peptide mapping software.
To eliminate redundancy in the obtained alignments, the following steps were taken: Only alignments with an alignment e value of less than 10− and 10−15 for genomic and transcriptomic data were considered.
All but one of duplicate alignments will be filtered to avoid redundancy caused by aligning once as individual reads and again as paired.
To create a redundancy-free reference sequence, we used BLAST, the Basic Local Alignment Search Tool [ 26], which has been used for removing redundancy together with the alignment program, CAP3, as well as for annotation [ 27].
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