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Duplicate alignment datasets were constructed containing either the toothed or baleen whale and the out-group taxa.
If an end-to-end alignment with at most k mismatches or k differences is found, it is then checked to determine if it is a duplicate alignment.
Duplicate alignment datasets were constructed containing either the two echolocating Yinpterochiroperan (R. ferrumequinum and M. lyra); the three non-echolocating Old World fruit bats (P. vampyrus, P. alecto and E. helvum) or the four echolocating Yangochiroptera (P. parnellii, M. lucifugus, Myotis davidii and Eptesicus fuscus).
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Duplicate alignments (reads aligning to the same genomic position) were removed using SAMtools [ 13].
In brief, duplicate alignments were removed from the aligned sequencing file (a correction for potential PCR artefacts), and the resulting output was loaded into an R workspace.
All but one of duplicate alignments will be filtered to avoid redundancy caused by aligning once as individual reads and again as paired.
Clusters of duplicate alignments were then extracted by grouping all reads with the same prefix and aligned position.
The first step in this stage is to filter all the duplicate alignments that are present in both lists, in order to remove redundant information.
After a second alignment of discordant chromPETs back to the human genome with more sensitive parameters, and excluding any "concordant" chromPET that map with the expected size and orientation, we processed all the resulting "discordant" chromPETs (including multiple mappings) using the HYDRA pipeline., Duplicate alignments were removed and then fed to the HYDRA pipeline with default parameters.
For that reason, it is common practice to remove PCR duplicates after alignment in whole-genome and whole-exome sequencing studies.
Each duplicated paired alignment (i.e., segments for which paralogous existed in the reference genome) was filtered using the reciprocal best hits option of the Fil program [ 48] under the following parameter set: coverage > 60% with respect to a reference gene and a percentage differential score cutoff < 10%.
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