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The derivatisation reagent employed for the derivatisation of anabolic steroids was a mixture of N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA), ammonium iodide and 2-mercaptoethanol (1000 2 6, v/w/v).
Specifically, the CO2 removal efficiency achieved in the absorption stage, the yield of alkali regeneration and CO2 uptake resulting for the regeneration stage, as well as the leaching behavior of the solid product are analyzed as a function of the type and concentration of the alkali reagent employed for the absorption reaction.
To explore this possibility, parasites were labeled with sulfo-NHS-Biotin, a membrane impermeable reagent employed to biotinylate external proteins.
The detection reagent employed was Amersham ECL Prime western Blotting Detection Reagent (GE Healthcare Life Sciences).
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All reagents employed were provided by Sigma Aldrich.
All the reagents employed in this study were of analytical grade and were used without any further purification.
Commercial reagents employed in the traditional preparation of MCM-41 are always expensive and toxic, such as tetraethylorthosilicate (TEOS) [22].
Other chemicals and reagents employed in the experiments were of analytical grade and were used as received from Sinopharm Chemical Reagent Co. , Ltd
The sources from which the reaction corpus was extracted were examined, and for each the reagents employed and the amounts thereof were identified.
While these factors are significantly influenced by probe design, they are also subject to the particular detection methodology and reagents employed.
That is to say, the reagents employed may be stated without reference to their amounts, such as in; "The reaction mixture was stirred at 25°C for 4 days and then diluted with ethyl acetate.
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