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The total carbohydrate present in liquid samples was estimated by using the anthrone reagent method.
Total RNA was extracted by the Trizol reagent method (Invitrogen, USA).
FAA was determined with the ninhydrin reagent method (Moore and Stein 1954).
The content of TSS was measured using the anthrone reagent method (Zhang et al. 2011).
Soluble sugars were estimated by the anthrone reagent method using glucose as the standard (Yemm and Willis [1954]).
Total phenolics in selected part of C. papaya were determined using the Folin Ciocalteau reagent method [33].
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Approximately 100 mg (±10 mg) of tissue from the renal cortex was homogenised in 1 ml of TRI-reagent, using the TRI-reagent method (Sigma Aldrich, St . Louis MO, USA).
Total RNA was isolated from cells using the TRI-REAGENT method (Molecular Research Center, Cincinnati, OH) according to the manufactureŕs instructions.
The amount of glucose generated by lactose hydrolysis was assessed by the Glucose oxidase-peroxidase mono-reagent method.
FOX-reagent method was used to determine this activity of the sample and the reference compound sodium pyruvate, as previously described [ 12].
RNA was isolated from Arabidopsis thaliana leaves (2 3 g) using the TriZol-reagent method according to the manufacturer's (Invitrogen, USA) instructions.
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