In contrast, the masked dilutions of the three positive control reagents performed as expected.
G H and X Z contributed reagents, performed experiments and analyzed data.
However, both transfection reagents performed similarly, but poorly, in their ability to transfect Schwann cells.
HL and LB contributed experimental reagents, performed experiments, and analyzed the data.
Both reagents performed similarly, although once again the dextramer gave much brighter staining than the corresponding tetramer.
Both reagents performed well when the TCR affinity was high (e.g. anti-viral CD8+ T cells), although the higher valency reagent always stained with higher intensity.
In organic synthesis, thallium III) salts, as thallium trinitrate or triacetate, are useful reagents performing different transformations in aromatics, ketones, olefins, among others.
However, due to the large size of quantum dot conjugates and their multivalency, these reagents perform poorly for one-to-one ligand-to-target binding [ 3].
This observation supports the premise that many common Elispot materials and reagents (e.g., plates, antibodies, spot development reagents) perform equally or similarly well; and that there are other factors which influence the outcome of the Elispot assay.
Although MALDI MS is capable of profiling small samples for qualitative information, adding labeling reagents, performing reactions, and mixing control and experimental samples often has limited the application of stable isotope labeling to many-fold larger samples.
