Sentence examples for reagent comprised from inspiring English sources

A pull-down reagent comprised of the Pak1-RBD was used as an affinity probe in immunohistochemistry experiments to demonstrate Rac activation in FFPE fixed mouse mammary tumors driven by Neu and TGFβ1 [26].

Briefly, 60 μL of sample was mixed with 180 μL of colour reagent comprised of 20 mL acid reagent (190 mL distilled water, 300 mL 85% phosphoric acid and 10 mL concentrated sulphuric acid), 1000 μL 2·5% diacetylmonoxime and 400 μL 0·25% thiosemicarbazide.

The reagent comprises of H2O2 and ferrous iron, and it generates hydroxyl radicals, which are reactive oxygen species (ROS) and oxidize organic pollutants including humic acid (Wang et al. 2012).

The FCAT reagent comprises four functional elements: a specific chemical reactivity group toward sulfhydryl groups; a linker that can incorporate the stable isotopes; a hydroxymethylbenzoic residue (base labile group) to cleave off a large part of the label before MS analysis; and a fluorescent tag for absolute quantification.

The BD FACSCount™ CD4 Reagent comprises a single tube which contains all reagents required for absolute CD4+ T-cell counts and CD4+ T-cell percentages.

The sample was dialysed against water, and then treated with Fenton reagent, comprising (added in this order; final concentrations quoted): 50 mM acetate buffer (Na+, pH 4.5), 10 mM H2O2, 1 μM CuSO4 and 10 mM ascorbic acid [ 4].

For miR-106b, miR-25, miR-93, s noRNA, and U6 snRNA, 4.0  μl of RT product was combined with 16.0  μl of PCR assay reagents (comprised of 5.0  μl of H2O, 10.0  μl of TaqMan 2X Universal PCR Master Mix, No AmpErase UNG, and 1.0  μl of TaqMan miRNA Assay) to generate a PCR of 20.0  μl of total volume.

For miRNAs and snoRNA, 4.0 ml of RT product was combined with 16.0 ml of PCR assay reagents (comprised of 5.0 ml of H2O, 10.0 ml of TaqMan 2X Universal PCR Master Mix, No AmpErase UNG, and 1.0 ml of TaqMan miRNA Assay) to generate a PCR of 20.0 μl of total volume.

Metalorganic titanium reagents comprising alkoxide and amide complexes were grafted onto the dehydrated MCM-48 samples in dry hexane to give (SiO 4−xTi(L x (L=NMe2, OiPr).

C2 and P21 forms were obtained using thrombin-treated protein with crystallization reagents comprising 100 mM phosphate/citrate pH 4.2, 30% PEG 300, and 100 mM citrate pH 4.7, 9% PEG 6000, respectively.

According to the method [44], a reagent comprising of 100 mM DMPD, 0.1 M acetate buffer (pH 5.25), and 0.05 M ferric chloride solution, which led to formation of DMPD radical, was freshly prepared and the reagent was equilibrated to an absorbance of 0.900 ± 0.100 at 505 nm.