Sentence examples for mutations of potential from inspiring English sources

Thus, on the basis of KAS III crystal structures the three-dimensional structure of BenQ was modeled and the predicted substrate-binding tunnel was altered by individual mutations of potential gatekeeping residues (H95A and M99A).

Mutations of potential trans-regulator binding sites were introduced in one of the truncated msn DNAs using the QuickChange site-directed mutagenesis kit from Stratagene.

During the last 20 years, numerous ACE mutations have been generated (more than 40) and among them, several where identified which had no enzymatic activity but measurable mRNA expression or ACE protein production; these were shown to be due to mutations of potential glycosylation sites.

For example, a Rec8 mutant with 24 point mutations of potential casein kinase 1 (CK1) and Cdc7 kinase (Dbf4 dependent kinase (DDK)) sites eliminated detectable phosphorylation of Rec8 protein and produced a discernable phenotype (22).

Luc-CCNJ 3′UTR was commercially constructed by RiboBio and the mutations of potential miR-205-binding sites on the Luc-CCNJ 3′UTR plasmid were performed by TransGen (TransGen Biotech Co., Ltd., Beijing, China).

We therefore introduced all five mutations of potential interest (QH, IV, DV, SN, NI) into PI5P4Kγ and PI5P4Kγ+ and both these mutant enzymes were completely resistant to NIH-12848 inhibition (Supplementary Figures S4A and S4B).

Overexpression of Y890A DG significantly restored podosomes to 70% of control levels, whereas mutation of other tyrosine residues or mutation of potential SH3 binding sites did not significantly affect podosome formation (Figure 7).

Mutation of potential T-box binding sites within the tbx-2 promoter resulted in a similar pattern of ectopic Ptbx-2:: gfp expression, and chromatin immunoprecipitation analyses show TBX-2 binds these sites in vivo.

Given the clinical context of these mutations in breast cancer, these mutations are of potential clinical benefit to the patient that may have implications for PIK3CA inhibitors that target the PI3K/AKT/mTOR pathway [ 21, 22].

He envisions a day when a device implanted in your body will be able to identify the first mutations of a potential tumour, or the genes of an invading bacteria.

cDNA constructs with mutations of the potential phosphorylation sites (Fig. 1A) within the cytoplasmic domain of NEP were generated and expressed in HEK293 cells.