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For every training sequence, 10 mutated sequences with t% mutations are created and added to the training set.
For each training sequence, N new mutated sequences with s% mutations are created and added to the training set.
For each sequence in the training set, M mutated sequences with p% mutations are created and added to the training set.
However, not all positively selected mutations are created equal.
During PCR reaction the Taq polymerase incorporates an adenine instead of a guanine and a cytosine instead of a thymine and non-reproducible C<>T and G<>A mutations are created [ 24- 26].
Since the HIV reverse transcriptase does not necessarily produce copies that are completely the same as the parent strain, mutations are created, some of which can result in ARV drug resistance.
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30 A topological file containing hybrid amino acids for all point mutations was created with a VMD plugin called Mutator, based on standard Chemistry at HARvard Molecular Mechanics (CHARMM) topology.
MEKK1 mutations were created by PCR mutagenesis.
Point mutations were created using the QuickChange site-directed mutagenesis kit (Stratagene, La Jolla, CA).
Single point mutations were created by site directed mutagenesis to create Aae MutL (H404A) and Aae MutL (R406A).
Point mutations were created by two-step PCR-based mutagenesis, and constructs were sequenced for amplification errors.
Related(20)
variations are created
mutations are implemented
mutations are accumulated
variants are created
mutations are incorporated
mutants are created
transfer are created
mutations are summarized
mutations are associated
mutations are known
mutations are seen
mutations are observed
mutations have created
mutations are spread
mutations are detected
mutations are mapped
mutations are kept
mutations are replicated
mutations are located
mutations are implicated
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