Sentence examples for mutation was set from inspiring English sources

A single point mutation was set via site-directed mutagenesis using the primers 5'-CCTAAAAACTGCATGGAGGCACAATATC-3' and 5'- GCAGTTTTTAGGACGACATCTGGCCTTAAAAG-3' leading to the amino acid E(1584)–K substitution on protein level (sequence confirmed by sequencing).

Haplotypes in control individuals were estimated using PHASE, and the relative position of the mutation was set within the haplotype.

The selection coefficient was set as 0.01, and the frequency of the advantageous mutation was set to range between 10% and 100%, in increments of 10%.

Although the threshold for calling a mutation was set to 2%% of base changes, not all base changes above 2%% were called as mutations.

Again, this was due to the fact that 16278T for haplogroup C4a3 was reported as missing in the haplotype even though this mutation was set in brackets in Phylotree.

The population size is 100 and the number of generations is 300; 2. The number of hill-climbing iterations for both crossover and mutation is set to 100; 3.

The overall distribution of mutations is set out in Figure 1.

Recurrent and unstable mutations are set in brackets by Phylotree [4].

The remaining five sampling years showed signs of bottlenecks in the vast majority of parameter combinations, except when the parameters p s (the fraction of mutations larger than a single step) and Δ g (the mean size of larger mutations) were set at their maximum (see Additional file 3 Table S2).

Here, the relative occurrence of a transitional mutation versus a transversional mutation (r) was set to be 4.0 (the expected ratio in the numbers of transitional and transversional mutations was 2.0).

The cut-off value defined for reliable mutation detection was set as a frequency of 5% mutant alleles.