Sentence examples for mutation was used from inspiring English sources

To produce mutated plasmids, site-directed mutation was used to introduce base substitution to the miR-133 binding site of luciferase reporters using Site-Directed Gene Mutagenesis Kit (Beyotime).

For double (or triple) mutations, the mutated vector for the first (or second) mutation was used as template.

This mutation was used to generate γ/ε-fc mice, in which a mutated ε subunit cDNA fragment is fused into the γ subunit gene by homologous recombination to replace AChRγ with functionally "silent" receptors (AChRγ/ε-fc) during embryonic development.

In next-generation sequencing by Mi-seq (illumina, Japan), genomic DNA including the mutation was used to amplify the region including gRNA2 and gRNA3 target sequences by PCR.

To generate the two isogenic cell lines FUS WT-GFP and P525L-GFP, a patient-derived iPSC clone from a female patient carrying a heterozygous FUS R521C mutation was used (Table 1).

Similar to (A), each N-terminal mutation was combined with a mutation I70A at a mid-tunnel region, which impairs the arrest partially (this mutation was used as it did not affect the electrophoretic mobility of MifM).

Two types of mutation are used, one random and the other 'intelligent' or beneficial.

Two genetic operations, which are arithmetic crossover and residual mutation, are used for GRS of the batch filter based on PF PFF batch filter).

In this study, either three or four iPSC lines from healthy controls and two lines from patients carrying the SOD1D90A mutation were used.

The "uniform mutation" is used as a mutation operator to avoid overly fast convergence problems.

Three basic operators (selection, crossover and mutation) are used to form the population.