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Exact(6)
In contrast with Col-0 fertilized by fbl17 mutants that only allowed 6 8% of transmission efficiency of the fbl17 mutant allele, the fie mutation raised the transmission efficiency of fbl17 to 27 32%.
CD measurements of the ellipticity at 219 nm as a function of temperature revealed that the Q40L mutation raised the Tm of Stx2B by 18°C relative to wild-type Stx2B, whereas the reciprocal mutation L41Q in Stx1B lowered the Tm by at least 15°C relative to wild-type Stx1B (Figure 6) (the unfolding transition for Stx1B was not completed by 95°C and thus could not be determined precisely).
The finding of the identical mutation raised the suspicion of cross contamination between cell lines.
In Bacillus sp. G1 a H47T mutation raised the ratio of γ-CD formed (Table 2), though the total γ-CD yield was only mildly enhanced.
Insertion of a point mutation within the AfFKS1 gene to generate a S678Y amino acid substitution resulted in an MEC of 4 μg/ml (Gardiner et al., 2005) and introducing a S678P mutation raised the MEC of caspofungin, anidulafungin and micafungin to >16 μg/ml (Rocha et al., 2007), compared to 0.25 μg/ml with the susceptible wild-type strain.
For instance, in cyts c6 and c6A, the glutamine at positions 52 and 51, respectively, were shown to raise the potential ∼100 mV, and in cyt c, the Tyr48Lys mutation raised the potential 117 mV; all these effects can be attributed to charge compensation in the heme pocket.
Similar(51)
Myriad executives have said that when a European laboratory finds a mutation in either of the two genes, 20 to 40 percent of the time it does not know if the mutation raises the cancer risk.
Thus, none of the infection parameters assayed were detectably affected by the X-ORF knockout mutation, raising the question whether DHBx expression plays a physiological role during in vivo infection with wild-type DHBV.
The greatest effect on muscle mass was observed in offspring of mothers homozygous for the Mstn mutation, raising the possibility that either myostatin itself or a downstream regulator may normally be transferred from the maternal to fetal circulations.
The mutation raises the net charge of the 100-residue N-terminal domain from +4 to +6.
It is likely that the Y254V mutation raises the free energy barrier of the transition state by disrupting coordination along the pathway of the translocation step.
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Justyna Jupowicz-Kozak
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