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If subsequent plasmid analysis reveals that clones lack the desired mutation, increase the digestion time to three hours.
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α is the multiplicative factor by which a mutator mutation increases the somatic mutation rate per cell generation, e.g. the magnitude of the genetic instability.
The N15D mutation increased the thermal stability of VPR by ∼ 3 °C, both in T50% and Tm.
"Every species leap [by H5N1] represents a new virus mutation, increasing the chance that one will become highly infectious to humans," says Dr Peter Horby, an epidemiologist at the World Health Organisation (WHO), in Hanoi.
Conversely, the T202C mutation increased the flexibility of loop A, enhancing the catalytic efficiency beyond that of the native enzyme.
The E22Q mutation increases the population with intact VGSN turn compared to the wild-type (WT) peptide.
The deletion mutation increased the sucrose consumption rate significantly when sucrose or sugarcane molasses was used as a carbon source.
Follow-up studies should clarify whether the Glu318Gly mutation increases the risk of cognitive decline in later life.
pH versus kcat profiles show that this mutation increases the acidity of the catalytic general acid Tyr55.
Each single mutation increased the half-inactivation temperature by 2 3 °C over that of the wild-type enzyme.
In addition to the conformational changes described above, we hypothesized that the G118V mutation increases the local hydrophobicity of the polypeptide, the structural consequences of which would be amplified by the presence of another valine residue at position 119.
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