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As shown in Figures 1B and C, the tetherin mutants with double substitutions inhibited the production of Lassa Z-induced VLP to a similar level as WT tetherin.

In 4 of 5 (80%) mutants with double amino acid substitutions proximal in structure to one another GeneSV was also correct in its predictions.

Upon examination of dfmr1 mmp1 double mutants, we found remarkable rescue of the dorsal tracheal deformation and breakage that characterizes mmp1 mutants, with double mutants never showing any detectable breaks in the dorsal trachea (0 breaks; n≥7; Fig. 6C).

5 additional mutants with double amino acid substitutions proximal in structure to each other were generated and in 80% of cases (4 of 5) GeneSV was correct in its predictions.

Similar(56)

Therefore, we could only compare triple mutants (N51I/C59R/S108N) with double mutants (N51I/S108N).

The purified TEVp mutants fused with double hexahistidine-tag at N and C terminus showed highest yield, solubility and cleavage efficiency.

First, there are 108 "double-double" tests that compare two double mutants with two other double mutants, holding the distribution of the mutant alleles constant, for example, ar·bs - as·br.

To identify genes required for survival upon both exogenous and endogenous oxidative stress, we tested an S. pombe collection of viable open reading frame deletion mutants with a double screening procedure.

For instance, mutants with a double knockout of the synthase genes (SN*, signal negative; the terms "signal negative" (SN) and "signal blind" (SB) were originally introduced by Diggle and associates [7] to designate ΔlasI and ΔlasR mutants, respectively, while we use the term for double lasI/rhlI and lasR/rhlR knock out mutants, respectively).

By auxanography analyses nearly two thirds of the auxotrophs were further characterized, including mutants with single, double and alternative nutritional requirements.

In this in silico model of E. coli metabolism, we again observe a strong MC for the wild type and low values for the mutants, with the double mutant exhibiting the lowest amount of coherence.

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