Sentence examples for mutants with two from inspiring English sources
To exclude any possible influence of the mutations on the protein structure and expression level we stained the various 221/HLA-G mutants with two conformational-dependent anti-MHC class I mAbs and observed staining of all mutants (Figures 2 and 3 left panel and data not shown).
We have also tested two additional mutants with two alleles of each (amn and FMR1).
Second, we calculated the set of non-standard interactions that contrast two double mutants with two other double mutants, while holding all of the component alleles constant.
The text in the subsection "Alternative definition of the slowest-flipping loop position for mutants with two slow-flipping loops" has been modified to clarify the usage of "antiparallel".
First, there are 108 "double-double" tests that compare two double mutants with two other double mutants, holding the distribution of the mutant alleles constant, for example, ar·bs - as·br.
For the K3, L85R, nEmrE, and nT28R mutants, which have two slow-flipping loops, the axes of this plot are defined so as to account for the positions of both loops, as described in the Alternative definition of the slowest-flipping loop position for mutants with two slow-flipping loops section of the 'Materials and methods'.
Conversely, mutants with six additional glutamates or aspartates appear to exist mostly as dimers and to have the same α-helical content as that of wild-type sulerythrin.
Overall, the expression level and solubility of the mutants with four IIAEK sequences in the variable regions IV and V were the best followed by II > III > I.
The resulting pRad-155 mutants with one or both Egr1 binding sites mutated were designated pRad-155m1, pRad-155m2 pRad-155m355m3.
Total RNAs were harvested from KREPA3-RKO derived cells that exclusively express TAP-tagged KREPA3 WT and mutants with one or both ZFs mutated and RT-PCR was performed to amplify A6 and MURF2 mRNAs (Fig. 5A).
However, significantly more mutants with eight amino acids are added in the products using (NNC 8 in comparison to those of (NNT 8.
