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All genes with double identifiers are indicated in Table 1 2 with an asterisk.
For a total of fifteen different adult tissues (Table S2), Fisher exact tests were carried out to examine overrepresentation of genes with double the expression level in that tissue compared to whole flies ('tissue-enriched expression') among the 1,498 differentially expressed genes.
The finding that the transcriptional activity of genes with double marks depends of the relative intensities of active and repressive marks suggested that there is not any dominant effect of H3K27me3 over H3K4me3 per se.
However, we have observed that most of the H3K4/K27me3 marked genes have stronger modification signal intensities of H3K27me3 than H3K4me3 and propose that the transcription activities of genes with double marks are reflected by the relative signal intensities of active and repressive marks.
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In an expression assay, Horie et al (1995) first reported that expression of the gene with triple repeat was higher than that of the gene with double repeat.
In contrast to the single-strand break associated gene set, genes associated with double-strand damage are enriched for gene ontology categories that include cell cycle regulation [see Additional file 2].
High levels of insulin similarly decrease the effectiveness of insulin, and mice overexpressing the insulin gene, with doubling and quadrupling insulin levels, fail to show change in weight or fasting glucose, while developing IR, postprandial hyperglycemia, and decreased insulin receptor binding.
To determine whether the bacterial-like MMR proteins encoded in the genome of H. salinarum NRC-1 were essential in maintaining the low genomic mutation rate we observed, we carried out targeted gene deletions of the mutS1A, mutS1B, mutL, and uvrD genes, with a double deletion of the mutS1A and mutS1B genes, using the background strains AK07 (Δura3) and CB08 (Δura3Δzim) [27], [27].
Among these 2,661 genes with enriched double (ERα and Pol-II) binding peaks, only 273 of them overlapped with 1,513 E2-induced genes in MCF7 show differential expression.
We also identified genes with high double connectivity in the letrozole-treated tumors as genes that are essential in an estrogen-depleted environment.
We first identified genes with high double connectivity in the untreated tumors as genes that are likely important for maintaining the tumor phenotype in an estrogen-rich environment.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com