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Clones of three independent mutants were enriched by seven rounds of selection and the mutant which was most frequently obtained (FA11) was further investigated.
The Nes-Cre and Pax8+/Cre Wt1co/co mutants were enriched for genes normally expressed in the Ren1-positive cells from the adult juxtaglomerular apparatus, suggesting that these cells maintain a relatively primitive character.
The enriched GO terms found among the negative interactions with the different DNA polymerase mutants were enriched for numerous pathways functioning in DNA replication, DNA damage checkpoint regulation, and various DNA repair pathways.
The mRNAs that were translationally stimulated in both rpl24b and eif3h mutants were enriched in ribosomal protein mRNAs (Additional file 1, also below) while the undertranslated ones had no detectable functional bias.
Similar(56)
The use of mutants in the small RNA biogenesis pathway may yet prove to be helpful in miRNA identification in rice, as some of these mutants are enriched for miRNAs, and analyses with high-throughput sequencing can be quite informative, as demonstrated recently in Arabidopsis [46].
Combined transcriptome analyses in lif2 and lhp1 mutants revealed that the class of deregulated genes in both mutants is enriched for stress-response genes.
Although there are a number of possible explanations for this memory profile, it is possible that this class of mutants is enriched for mice with abnormal hippocampal function.
Neither of these mutants was enriched in spines.
Genes upregulated in MZ eomesa mutants are enriched for expression in the ectoderm, whilst downregulated genes are enriched for expression in mesendoderm.
The genes that show SSI with the As- or P-sets and the arsenite-sensitive gene deletion mutants are enriched for similar functions including protein fate (folding, modification, destination), cell cycle, cellular differentiation and cell fate (Fig. 5E).
The molecular functions of genes showing lower expression in the mutant were enriched for those having hydrolytic activity.
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