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Back in 1978, my class was the first undergraduate genetics class to use E. coli in addition to Drosophila, so we were promised that any mutants we developed would bear our name.
To detect comprehensive CD20 molecules including the resistant mutants, we developed a novel monoclonal antibody that recognizes the N-terminal cytoplasm region of CD20 molecule.
To provide resources for depositing and retrieving information on M. loti mutants, we developed a relational database, RhizoGenes (http://www.kazusa.or.jp/rhizobase/Mesorhizobium/genes2/index.html).html
To confirm reduced methylation at position C2268 in nuclear 25S rRNA in nsun5 mutants, we developed a restriction enzyme digestion of PCR products using a dCAPs (derived cleaved amplified polymorphic sequences) primer derived from BS treated 25S rRNA.
To more precisely quantify the thrashing behavior of our vt mutants, we developed software tools (SwimR; see Materials and Methods, J. A. Hardaway and J. Wang, unpublished data) that can provide for a more detailed kinetic analysis of individual animals that may reveal patterns of behavior not readily detected in population averages (Table 1).
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To decipher the inheritance behavior of ygl138 mutant, we developed an F2 population between ygl138 and cultivar rice Minghui 63 and found that the F1 generation exhibited normal green leaf phenotype.
Based on this mutant, we developed a novel comparison system that can minimize the influence of genetic backgrounds and growing environment.
By exploiting the nonstandard recombination activity exhibited by a phiC31 integrase mutant, we developed a rapid and inexpensive method for isolating landing sites that exhibit desired expression properties.
Using this mutant, we developed a novel comparison system, and thereby enzymes or biochemical pathways responsible for chalkiness formation were identified, including pathways of carbohydrate metabolism, protein synthesis, folding, and degradation, and ROS scavenging.
Supporting evidence is that low-level intergenic transcripts that are dependent on Pol V can be detected in vivo by using RT-PCR; Pol V physically associates with these loci and production of the intergenic RNAs is abolished in the NRPE1 Metal A site mutant lines we developed in the current study [7].
To create and maintain in collection mutant aphids, we developed a dedicated protocol.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com